2026-10-12T05:51:35Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33228621 2026-09-21T01:38:59Z category_24733 portal_886 item_type_3 month_year_09_2026

Supporting data for "Adiponectin Deficiency Promotes Microglia Activation and Exacerbates Tau-Mediated Neurodegeneration" Zifei Zhang (12989555) Central nervous system Alzheimer's disease Neurodegeneration Tauopathies Microglia Neuroinflammation Adiponectin <p dir="ltr">Tau pathology is a pathological hallmark of neurodegenerative diseases, including Alzheimer’s disease (AD), and its progression is critically driven by microglia-mediated neuroinflammation. While the adipokine adiponectin (APN) exerts anti-inflammatory effects, whether it alleviates tau-mediated neurodegeneration remains unknown. In this study, we first investigated the clinical relationship between BBB-penetrant APN levels, cognitive performance, and microglial activation in cohorts of patients with AD and non-AD. We generated a novel APN-deficient tauopathy mouse model (Tau^P301S;<i>APN</i>^<em>-/-</em>) by crossbreeding APN knockout (<i>APN</i>^{<em>−/−</em>}) and human tau P301S mutation (Tau^P301S) mice. We aimed to study: 1) whether APN deficiency recapitulates the neurodegeneration and microglial activation characteristic of tauopathies; 2) whether APN deficiency exacerbates tau-mediated neurodegeneration in Tau^P301S mice; 3) whether this deficiency enhances microglia-mediated neuroinflammation in Tau^P301S mice; and 4) whether pharmacological activation of the APN receptors can mitigate tau-mediated neurodegeneration by restoring microglial homeostasis. Animal studies included behavioural tests (open field and novel object recognition), immunofluorescent staining, Golgi staining, single-nucleus RNA sequencing, and ELISA. In vitro studies included microglial phagocytosis, lysosomal activity, and MTT assay.</p> 2026-09-21T01:38:59Z Dataset Dataset 10.25442/hku.33228621.v1 https://figshare.com/articles/dataset/Supporting_data_for_Adiponectin_Deficiency_Promotes_Microglia_Activation_and_Exacerbates_Tau-Mediated_Neurodegeneration_/33228621 CC BY-NC 4.0 Embargoed