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Supplementary Information: Colloidally dispersed nanoparticles: a convergent evolutionary approach for the secretion of apparently soluble melanin Deepesh Nagarajan (23494372) Phylogeny and comparative analysis Mycology Colloid and surface chemistry colloidal particle solutions Fungal Melanin Biosynthesis convergent evolutionary strategies Supplementary informationDataset S1:SEM_data: Supplementary data for Figure 2. This dataset contains all SEM micrographs collected for colonies of Glyomastix polychroma (VIG 6138 / NFCCI 6138), Stachybotrys chlorohalonatus (VIG 2586), Curvularia sp. (VIG 2862), and Pseudopithomyces sp. (VIG 728). All cultures were grown on Sabouraud dextrose agar for 30 days. Cultures were refrigerated prior to environmental SEM visualization.Dataset S2:TEM_data: Supplementary data for Figure 6 and Figure 7. This dataset contains TEM micrographs depicting individual nanoparticles for the following melanin classes: L-DOPA melanin (soluble), L-DOPA melanin (insoluble), colloidal melanin from Glyomastix polychroma (VIG 6138 / NFCCI 6138), colloidal melanin from Stachybotrys chlorohalonatus (VIG 2586), colloidal melanin from Curvularia sp. (VIG 2862), and colloidal melanin from textitPseudopithomyces sp. (VIG 728). R and bash scripts for statistical analysis and data visualization are also provided.Dataset S3:LC-MS_data Supplementary data for Table 2. This dataset contains LC-MS reports for acid-hydrolyzed melanin obtained from Glyomastix polychroma (VIG 6138 / NFCCI 6138), Stachybotrys chlorohalonatus (VIG 2586), Curvularia sp. (VIG 2862), and Pseudopithomyces sp. (VIG 728) are provided. Spreadsheets and R scripts for data analysis and statistical analysis are also provided.Dataset S4:SEM-EDS_data Supplementary data for Figure 5 and Table 3 are provided. nd Figure 7. This dataset contains SEM-EDS reports containing the elemental compositions of colloidal melanin obtained from Glyomastix polychroma (VIG 6138 / NFCCI 6138), Stachybotrys chlorohalonatus (VIG 2586), Curvularia sp. (VIG 2862), and Pseudopithomyces sp. (VIG 728). Spreadsheets, R and bash scripts for data analysis and data visualization are also provided.Dataset S5:annotated_gene_sequences: Supplementary data for Table 5, Figure 8, and Supplementary Tables S1-S4. This dataset contains genes identified from whole-genome sequencing (WGS) and annotated as putatively involved in melanin biosynthetic pathways for Glyomastix polychroma (VIG 6138 / NFCCI 6138), Stachybotrys chlorohalonatus (VIG 2586), Curvularia sp. (VIG 2862), and Pseudopithomyces sp. (VIG 728). Functional annotations were assigned based on EggNOG-marker and KEGG pathway analysis predictions and homology to known L-DOPA, DHN, and HGA melanin biosynthetic enzymes, with predicted inhibitor targets mentioned where applicable. Gene data is provided in .fasta format. Scripts in Python, R, and bash are provided for data analysis, statistical analysis, and data visualization. Raw data used to construct the phylogenetic tree in Figure 8B is provided as a .meg (MEGA6) file.Text S1: Multilocus molecular identification and phylogenetic analysis for Glyomastix polychroma (VIG 6138 / NFCCI 6138).Text S2: Species identification of Stachybotrys chlorohalonatus (VIG 2586).Text S3: ITS sequences for all strains examined in this study: Glyomastix polychroma (VIG 6138 / NFCCI 6138), Stachybotrys chlorohalonatus (VIG 2586), Curvularia sp. (VIG 2862), Pseudopithomyces sp. (VIG 728).Table S1: Supplementary data for Table 5. Genes from Glyomastix polychroma (VIG 6138 / NFCCI 6138) identified from whole-genome sequencing (WGS) and annotated as putatively involved in melanin biosynthetic pathways. Functional annotations were assigned based on EggNOG-mapper and KEGG pathway analysis predictions and homology to known L-DOPA, DHN, and HGA melanin biosynthetic enzymes, with predicted inhibitor targets mentioned where applicable.Table S2: Supplementary data for Table 5. Genes from Stachybotrys chlorohalonatus (VIG 2586) identified from whole-genome sequencing (WGS) and annotated as putatively involved in melanin biosynthetic pathways. Functional annotations were assigned based on EggNOG-mapper and KEGG pathway analysis predictions and homology to known L-DOPA, DHN, and HGA melanin biosynthetic enzymes, with predicted inhibitor targets mentioned where applicable.Table S3: Supplementary data for Table 5. Genes from Curvularia sp. (VIG 2862) identified from whole-genome sequencing (WGS) and annotated as putatively involved in melanin biosynthetic pathways. Functional annotations were assigned based on EggNOG-mapper and KEGG pathway analysis predictions and homology to known L-DOPA, DHN, and HGA melanin biosynthetic enzymes, with predicted inhibitor targets mentioned where applicable.Table S4: Supplementary data for Table 5. Genes from Pseudopithomyces sp. (VIG 728) identified from whole-genome sequencing (WGS) and annotated as putatively involved in melanin biosynthetic pathways. Functional annotations were assigned based on EggNOG-mapper and KEGG pathway analysis predictions and homology to known L-DOPA, DHN, and HGA melanin biosynthetic enzymes, with predicted inhibitor targets mentioned where applicable. 2026-09-20T12:10:30Z Dataset Dataset 10.6084/m9.figshare.33088712.v2 https://figshare.com/articles/dataset/Supplementary_Information_Colloidally_dispersed_nanoparticles_a_convergent_evolutionary_approach_for_the_secretion_of_apparently_soluble_melanin/33088712 CC BY 4.0