2026-10-06T13:58:16Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33050051 2026-09-24T21:05:04Z category_24178 category_24148 category_24169 category_24403 category_28885 portal_295 item_type_3 month_year_09_2026

Near-isotropic, aligned volume electron microscopy (eFIB-SEM) of mosquito stylet (jrc_mosquito-stylet-6) CellMap Project Team (14322654) Yoonji Kim (7512554) Wei-Ping Li (14691662) Zhiyuan Lu (11450573) H. Amalia Pasolli (15357277) Stephan Preibisch (12975014) Wei Qiu (9903656) Eric T. Trautman (6733172) Leslie Voshall (24611538) Aubrey Weigel (9302851) Biochemistry and cell biology not elsewhere classified Cellular interactions (incl. adhesion, matrix, cell wall) Structural biology (incl. macromolecular modelling) Animal cell and molecular biology Image processing FIB-SEM Focused Ion Beam-Scanning Electron Microscopy Electron Microscopy Segmentations machine learning-based organelle membranes manual annotation <p dir="ltr"><b>Sample</b>: Stylet from an adult female Aedes aegypti mosquito.<br><br><b>Sample Description</b>: A dataset featuring isotropic 3D electron microscopy data of a stylet from an adult female <i>Aedes aegypti</i> mosquito (jrc_mosquito-stylet-6). The stylet was dissected at approximately three-quarters length and immediately fixed in 2% glutaraldehyde and 4% formaldehyde in sodium cacodylate buffer. It was processed using a reduced osmium-thiocarbohydrazide-osmium protocol with en bloc uranyl acetate staining, dehydrated through an acetone series, embedded in Eponate 12 resin, and polymerized at 60°C for two days. The sample was provided by Hilda Amalia Pasolli and Adriana Rosas (Vosshall Lab, HHMI / The Rockefeller University), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia).<br><br><b>Protocol</b>: The stylet was dissected at approximately three-quarters length and immediately fixed in 2% glutaraldehyde and 4% formaldehyde in sodium cacodylate buffer. It was processed using a reduced osmium-thiocarbohydrazide-osmium protocol with en bloc uranyl acetate staining, dehydrated through an acetone series, embedded in Eponate 12 resin, and polymerized at 60°C for two days. The selected sample was imaged by FIB-SEM at 8 nm isotropic resolution.<br><br><b>Contributions</b>: Sample provided by Hilda Amalia Pasolli (The Rockefeller University), prepared for imaging by Zhiyuan Lu (HHMI/Janelia) and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).<br><br><b>Acquisition ID</b>: jrc_mosquito-stylet-6<br><br><b>Voxel size (nm)</b>: 8 x 8 x 8 (x, y, z)<br><br><b>Data dimensions (µm)</b>:<b> </b>50.88 x 44.23 x 295.02 (x, y, z)<br><br><b>Imaging start date</b>: 2025-08-12<br><br><b>Imaging duration (days)</b>: 55<br><br><b>Landing energy (eV)</b>: 1200<br><br><b>Imaging current (nA)</b>: 2.00<br><br><b>Scanning speed (MHz)</b>: 0.500<br><br><b>Dataset URL</b>: <a href="https://data.janelia.org/IVV6Rg" target="_blank" rel="noreferrer">s3://janelia-cosem-datasets/jrc_mosquito-stylet-6/jrc_mosquito-stylet-6.zarr/recon-1/em/</a><br><br><b>Visualization Website</b>: <a href="https://openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6" target="_blank" rel="noreferrer noopener">https://openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6</a><br><br><b>Publication</b>:<b> </b>Xu et al., 2017</p> 2026-09-24T21:05:04Z Dataset Dataset 10.25378/janelia.33050051.v1 https://figshare.com/articles/dataset/Near-isotropic_aligned_volume_electron_microscopy_eFIB-SEM_of_mosquito_stylet_jrc_mosquito-stylet-6_/33050051 CC BY 4.0