2026-10-14T21:02:40Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33006187 2026-09-23T12:35:15Z category_7 category_8 category_15 category_16 category_39 category_734 category_132 category_134 category_135 portal_87 item_type_3 month_year_09_2026

A wire-obstacle model has no effect on typical lesions and positive re-isolations after an infection with Enterococcus cecorum in SPF broilers Thijs T. M. Manders (25097296) Arjan J. Baijense (24407470) Lonneke Vervelde (181449) Jeanine Wiegel (20589330) Medicine Microbiology Neuroscience Physiology Ecology Biological Sciences not elsewhere classified Infectious Diseases Virology Computational Biology Enterococcus cecorum wire-obstacle SPF broilers bacterial chondronecrosis and osteomyelitis mechanical stress pericarditis spondylitis Enterococcus cecorum lesions include bacterial chondronecrosis with osteomyelitis (BCO) of the femur and free thoracic vertebrae. Mechanical stress can be applied using wire-obstacle models and has been suggested as a predisposing factor for BCO. Here, the effect of a wire-obstacle model was assessed following inoculation with E. cecorum. Two groups SPF broilers were orally inoculated at day of hatch with 107.6 colony-forming units of E. cecorum in peptone physiological saline (PPS) per bird. In one group, a wire-obstacle was placed between the feed and water. This obstacle had a 66% slope, a height of 30 cm and was covered with iron mesh. Additionally, two control groups were inoculated with PPS, with an obstacle placed in one group. At 3, 7, 10, 14, 21, 28, 35, and 42 days post-inoculation, 20 birds from the E. cecorum-inoculated groups and eight birds from the PPS-inoculated groups were euthanized and macroscopically examined. Samples from spleen, femur and free thoracic vertebrae were subjected to bacteriological examination. No significant differences in the number of birds with typical lesions or positive reisolations were observed between the E. cecorum-inoculated groups with and without the obstacle. Typical lesions were observed in 58 of the 320 inoculated birds, with pericarditis being the most common lesion (46 birds). Femoral BCO was observed in seven birds. E. cecorum was reisolated in 45 of the 320 inoculated birds and in none of the controls. A wire-obstacle model does not predispose for BCO in an E. cecorum model using SPF broilers. 2026-09-23T12:35:15Z Dataset Dataset 10.6084/m9.figshare.33006187.v2 https://figshare.com/articles/dataset/A_wire-obstacle_model_has_no_effect_on_typical_lesions_and_positive_re-isolations_after_an_infection_with_i_Enterococcus_cecorum_i_in_SPF_broilers/33006187 CC BY 4.0