2026-10-12T03:20:48Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32863052 2026-10-01T16:03:46Z portal_1172 item_type_8 month_year_10_2026

A bioactive peptide from the skin secretion of the Chinese Torrent Frog, Amolops wuyiensis Ziyan Huang (3863497) PUREID: 157598011 Natural products, as rich and varied resources for the discovery of novel drugs, have been essential for human healthcare systems throughout history. A variety of bioactive components with unique structures and functions which isolated from the skin secretion, especially the peptides, have enormous potential for medical purposes. <br>Here, a Kunitz-type peptide was identified from the Chinese Torrent Frog, Amolops wuyiensis by molecular cloning. Then the mature peptide was successfully synthesised by Tribute® 2-channel peptide synthesiser. Reversed-phase high performance liquid chromatography (RP-HPLC) was used for purification and matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) MS was used to confirm its molecular mass. This peptide was found to show relatively strong trypsin inhibition with a Ki value of 2.331 µM with weak hemolysis at a concentration up to 512μM. The peptide was ineffective against the gram-positive bacterium S.aureus, gram-negative bacterium E.coli and pathogenic yeast C.albicans. Thus, QUB-1402 could expand the potential drug discovery and development pipeline directed towards alleviation of serine protease-mediated pathologies.<br> 2026-10-01T16:03:46Z Text Thesis 10.17034/32863052.v1 https://figshare.com/articles/thesis/A_bioactive_peptide_from_the_skin_secretion_of_the_Chinese_Torrent_Frog_Amolops_wuyiensis/32863052 All Rights Reserved