2026-10-11T09:54:42Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32863025 2026-10-01T16:04:01Z portal_1172 item_type_8 month_year_10_2026

QUB-1439: A bioactive peptide from the frog skin secretion of Hylarana guentheri Ziyu Ling (24161547) PUREID: 157526316 Antimicrobial peptides (AMPs) are a sufficient and diverse group of molecules that are derive from various invertebrate, plant and animal species. AMPs possess a variety of biological activity, such as anticancer, antivirus and so on. Nowadays, a growing number of AMPs are considered as promising candidate of new drug. <br><br>This thesis focused on the antimicrobial peptide (QUB-1439) which was isolated from the skin secretion of Hylarna guentheri. It is the first time to identify the biosynthetic precursor encoding cDNA of QUB-1439 from the skin secretion-derived cDNA library though the mature peptide sequence, FLPLLFGAISHLL-NH2, has been identified before. Subsequently, QUB-1439 was synthesised by Tribute peptide synthesiser. Then, MALDI-TOF was used to check the molecular weight of the synthetic peptide. The crude peptide was purified by RP-HPLC. Finally, a series assays were performed to test the bioactivities of purified QUB-1439, including antimicrobial assay, anticancer assay and haemolysis assay. The results showed that QUB-1439 has ability to inhibit the growth of Gram-positive bacteria, the MIC of QUB-1439 against S. aureus is 16 μM. QUB-1439 does not exhibit toxicity and it has a weak effect on inhibition of cancer cell growth. Because QUB-1439 has these promising properties, it could be developped into a novel drug.<br> 2026-10-01T16:04:01Z Text Thesis 10.17034/32863025.v1 https://figshare.com/articles/thesis/QUB-1439_A_bioactive_peptide_from_the_frog_skin_secretion_of_Hylarana_guentheri/32863025 All Rights Reserved