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oai:figshare.com:article/32862548 2026-10-01T16:07:35Z portal_1172 item_type_8 month_year_10_2026

A bioactive peptide from the skin of the Chinese Torrent Frog, <i>Amolops wuyiensis</i> Kelei Lu (23479270) PUREID: 134674832 Peptides derived from frog skin secretion have great potential as drug leads as they exhibit many bioactive functions. In this study, the precursor peptide of Lividin-AW was identified from the skin secretion of the Chinese torrent frog (Amolops wuyiensis) by using ‘shotgun’ cloning and its encoded mature peptide, sequenced as AVPLIYNRPGVYVTKRPK-NH2, was synthesised by Fmoc-solid phase peptide synthesis. The structure of the synthetic peptide was confirmed by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry and purified by reversed-phase high performance liquid chromatography (RP-HPLC). To determine the bioactive function of Lividin-AW, a series of bioactivity assays, including antimicrobial assays, cell viability assays, a trypsin inhibition assay and a haemolysis assay, were conducted and the results showed that Lividin-AW exhibited weak antimicrobial activity against Candida albicans (MIC=128µM), Staphylococcus aureus (MIC=512 µM) and Escherichia coli (MIC=512 µM), with negligible haemolytic activity, but neither trypsin inhibition activity nor anticancer activity were detected. 2026-10-01T16:07:35Z Text Thesis 10.17034/32862548.v1 https://figshare.com/articles/thesis/A_bioactive_peptide_from_the_skin_of_the_Chinese_Torrent_Frog_i_Amolops_wuyiensis_i_/32862548 All Rights Reserved