2026-10-09T12:04:23Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32816456 2026-09-22T21:25:16Z category_30563 category_30881 category_30638 category_30827 category_30650 category_30992 category_31001 category_33752 category_33791 category_33797 category_33935 category_33956 category_31208 category_31061 portal_1059 item_type_3 month_year_09_2026

Investigations into factors that drive the emergence of novel influenza reassortants in pigs under field conditions Montse Torremorell (24298865) Joaquin Alvarez-Norambuena (24304854) My Yang (24304855) Agricultural, veterinary and food sciences Biological sciences Animal production Veterinary sciences Animal protection (incl. pests and pathogens) Evolutionary biology Biological adaptation Health sciences Epidemiology Disease surveillance Public health Public health not elsewhere classified Biomedical and clinical sciences Genomics Influenza Pigs reassortants reassortment event reassortment analyses In order to gain insight into the level of genetic diversity and reassortment occurring in pigs, we sampled pigs throughout their production cycle from commercial farms known to be infected with multiple subtypes of influenza virus. Multiple influenza virions were isolated from single pigs by plaque purification, their genome amplified using MBTuni primers, cDNA libraries prepared using Nextera DNA XT sample preparation kit, their whole genome sequenced by Ilumina NextSeq platform and analyzed to identify whether pigs harbored one or more distinct types of influenza viruses (i.e genotypes). A blood sample was also collected from the pigs to evaluate the pigs’ immune status against the influenza viruses present in the farm. Sera was tested by the inhibition of hemaglutination test. The protocols for animal sampling and testing were approved by the University of Minnesota IACUC and IBC. Information on pigs’ age, production stage and cohort was obtained. In total we investigated three cohorts of pigs, from weaning to near market, that were mixed at weaning and originated from three breeding herds. We sampled 720 pigs from which 62 influenza infected pigs yielded 428 viral plaques which after further characterization yielded 173 complete genomes and 237 incomplete genomes. The serological analysis using the hemagglutination inhibition test to evaluate levels of protective immunity against the distinct influenza viruses known to be present in the herds were conducted against four distinct types of influenza viruses including representatives of the H1 and H3 subtypes. 2026-09-22T21:25:16Z Dataset Dataset 10.13020/6VM1-7R16 https://figshare.com/articles/dataset/Investigations_into_factors_that_drive_the_emergence_of_novel_influenza_reassortants_in_pigs_under_field_conditions/32816456 CC BY 4.0