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oai:figshare.com:article/32805719 2026-10-01T16:13:46Z portal_1172 item_type_8 month_year_10_2026

Development and testing of a proof of concept Cathepsin S ProteaseTag activity-based immunoassay Caitlin Taylor (4238095) PUREID: 687891682 Cathepsin S imunnoassay development drug discovery cysteine proteases Cystic Fibrosis (CF) acute respiratory distress syndrome inhibitors activity-based probes Cathepsin S (CatS) is an elastolytic cysteine protease involved in antigen presentation and is reportedly upregulated in diseases including rheumatoid arthritis (RA), acute respiratory distress syndrome (ARDS), chronic obstructive pulmonary disease (COPD), and cystic fibrosis (CF). Studies have shown increased CatS activity correlates with reduced lung function. This thesis developed highly potent and selective CatS inhibitors that were functionalised into activity-based probes (ABPs) for use in an immunoassay capable of detecting active CatS in complex patient samples. The resulting assay successfully detected CatS in sputum from CF and COPD patients, highlighting its potential for earlier diagnosis and intervention in chronic lung diseases.<br><br><i>Thesis is embargoed until 31 July 2031.</i> 2026-10-01T16:13:46Z Text Thesis 10.17034/32805719.v1 https://figshare.com/articles/thesis/Development_and_testing_of_a_proof_of_concept_Cathepsin_S_ProteaseTag_activity-based_immunoassay/32805719 All Rights Reserved Open Access after 2031-07-31