2026-07-15T18:17:33Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32791977 2026-06-26T06:48:08Z category_24328 category_24337 portal_549 item_type_3 month_year_06_2026

10.5522/04/32791977.v1 https://figshare.com/articles/dataset/Elucidation_of_the_growth_phenotypes_of_82_strains_from_the_i_Halomonas_i_genus/32791977 https://ndownloader.figshare.com/files/65866995 https://ndownloader.figshare.com/files/65866962 https://ndownloader.figshare.com/files/65866974 https://ndownloader.figshare.com/files/65866965 https://ndownloader.figshare.com/files/65866989 https://ndownloader.figshare.com/files/65866980 https://ndownloader.figshare.com/files/65866986 https://ndownloader.figshare.com/files/65866956 https://ndownloader.figshare.com/files/65867001 https://ndownloader.figshare.com/files/65866968 https://ndownloader.figshare.com/files/65867031 https://ndownloader.figshare.com/files/65866959 https://ndownloader.figshare.com/files/65867025 https://ndownloader.figshare.com/files/65867004 https://ndownloader.figshare.com/files/65866983 https://ndownloader.figshare.com/files/65866971 https://ndownloader.figshare.com/files/65866992 https://ndownloader.figshare.com/files/65866998 https://ndownloader.figshare.com/files/65866977 https://ndownloader.figshare.com/files/65867028 https://ndownloader.figshare.com/files/65867022 https://ndownloader.figshare.com/files/65867040 https://ndownloader.figshare.com/files/65867007 https://ndownloader.figshare.com/files/65867070 https://ndownloader.figshare.com/files/65867052 https://ndownloader.figshare.com/files/65867013 https://ndownloader.figshare.com/files/65867034 https://ndownloader.figshare.com/files/65867010 https://ndownloader.figshare.com/files/65867016 https://ndownloader.figshare.com/files/65867043 https://ndownloader.figshare.com/files/65867046 https://ndownloader.figshare.com/files/65867055 https://ndownloader.figshare.com/files/65867058 https://ndownloader.figshare.com/files/65867037 https://ndownloader.figshare.com/files/65867019 https://ndownloader.figshare.com/files/65867049 https://ndownloader.figshare.com/files/65867064 https://ndownloader.figshare.com/files/65867061 https://ndownloader.figshare.com/files/65867067 https://ndownloader.figshare.com/files/65867073 https://ndownloader.figshare.com/files/65867076 https://ndownloader.figshare.com/files/65867109 https://ndownloader.figshare.com/files/65867088 https://ndownloader.figshare.com/files/65867094 https://ndownloader.figshare.com/files/65867097 https://ndownloader.figshare.com/files/65867091 https://ndownloader.figshare.com/files/65867100 https://ndownloader.figshare.com/files/65867085 https://ndownloader.figshare.com/files/65867106 https://ndownloader.figshare.com/files/65867079 https://ndownloader.figshare.com/files/65867082 https://ndownloader.figshare.com/files/65867103 https://ndownloader.figshare.com/files/65867115 https://ndownloader.figshare.com/files/65867121 https://ndownloader.figshare.com/files/65867130 https://ndownloader.figshare.com/files/65867118 https://ndownloader.figshare.com/files/65867136 https://ndownloader.figshare.com/files/65867112 https://ndownloader.figshare.com/files/65867124 https://ndownloader.figshare.com/files/65867139 https://ndownloader.figshare.com/files/65867127 https://ndownloader.figshare.com/files/65867133 https://ndownloader.figshare.com/files/65867142 https://ndownloader.figshare.com/files/65867145 https://ndownloader.figshare.com/files/65867151 https://ndownloader.figshare.com/files/65867154 https://ndownloader.figshare.com/files/65867157 https://ndownloader.figshare.com/files/65867160 https://ndownloader.figshare.com/files/65867172 https://ndownloader.figshare.com/files/65867169 https://ndownloader.figshare.com/files/65867184 https://ndownloader.figshare.com/files/65867178 https://ndownloader.figshare.com/files/65867181 https://ndownloader.figshare.com/files/65867196 https://ndownloader.figshare.com/files/65867175 https://ndownloader.figshare.com/files/65867166 https://ndownloader.figshare.com/files/65867163 https://ndownloader.figshare.com/files/65867187 https://ndownloader.figshare.com/files/65867193 https://ndownloader.figshare.com/files/65867199 https://ndownloader.figshare.com/files/65867190 https://ndownloader.figshare.com/files/65867202 https://ndownloader.figshare.com/files/65867226 https://ndownloader.figshare.com/files/65867217 https://ndownloader.figshare.com/files/65867208 https://ndownloader.figshare.com/files/65867205 https://ndownloader.figshare.com/files/65867223 https://ndownloader.figshare.com/files/65867211 https://ndownloader.figshare.com/files/65867214 https://ndownloader.figshare.com/files/65867220 https://ndownloader.figshare.com/files/65867229 https://ndownloader.figshare.com/files/65867232 https://ndownloader.figshare.com/files/65867238 https://ndownloader.figshare.com/files/65867235 https://ndownloader.figshare.com/files/65867247 https://ndownloader.figshare.com/files/65867241 https://ndownloader.figshare.com/files/65867250 https://ndownloader.figshare.com/files/65867244 https://ndownloader.figshare.com/files/65867253 https://ndownloader.figshare.com/files/65867262 https://ndownloader.figshare.com/files/65867265 https://ndownloader.figshare.com/files/65867256 https://ndownloader.figshare.com/files/65867259 https://ndownloader.figshare.com/files/65867274 https://ndownloader.figshare.com/files/65867283 https://ndownloader.figshare.com/files/65867271 https://ndownloader.figshare.com/files/65867268 https://ndownloader.figshare.com/files/65867277 https://ndownloader.figshare.com/files/65867280 https://ndownloader.figshare.com/files/65867286 https://ndownloader.figshare.com/files/65867289 https://ndownloader.figshare.com/files/65867292 https://ndownloader.figshare.com/files/65867307 https://ndownloader.figshare.com/files/65867313 https://ndownloader.figshare.com/files/65867301 https://ndownloader.figshare.com/files/65867298 https://ndownloader.figshare.com/files/65867310 https://ndownloader.figshare.com/files/65867295 https://ndownloader.figshare.com/files/65867316 https://ndownloader.figshare.com/files/65867304 https://ndownloader.figshare.com/files/65867325 https://ndownloader.figshare.com/files/65867319 https://ndownloader.figshare.com/files/65867322 https://ndownloader.figshare.com/files/65867334 https://ndownloader.figshare.com/files/65867337 https://ndownloader.figshare.com/files/65867328 https://ndownloader.figshare.com/files/65867331 https://ndownloader.figshare.com/files/65867340 https://ndownloader.figshare.com/files/65867343 https://ndownloader.figshare.com/files/65867346 https://ndownloader.figshare.com/files/65867349 https://ndownloader.figshare.com/files/65867352 https://ndownloader.figshare.com/files/65867355 https://ndownloader.figshare.com/files/65867358 https://ndownloader.figshare.com/files/65867361 https://ndownloader.figshare.com/files/65867364 https://ndownloader.figshare.com/files/65867367 https://ndownloader.figshare.com/files/65867370 https://ndownloader.figshare.com/files/65867373 https://ndownloader.figshare.com/files/65867382 https://ndownloader.figshare.com/files/65867379 https://ndownloader.figshare.com/files/65867388 https://ndownloader.figshare.com/files/65867391 https://ndownloader.figshare.com/files/65867385 https://ndownloader.figshare.com/files/65867376 https://ndownloader.figshare.com/files/65867397 https://ndownloader.figshare.com/files/65867394 https://ndownloader.figshare.com/files/65867403 https://ndownloader.figshare.com/files/65867400 https://ndownloader.figshare.com/files/65867409 https://ndownloader.figshare.com/files/65867406 https://ndownloader.figshare.com/files/65867412 https://ndownloader.figshare.com/files/65867415 https://ndownloader.figshare.com/files/65867418 https://ndownloader.figshare.com/files/65867421 https://ndownloader.figshare.com/files/65867424 https://ndownloader.figshare.com/files/65867427 https://ndownloader.figshare.com/files/65867430 https://ndownloader.figshare.com/files/65867433 https://ndownloader.figshare.com/files/65867436 https://ndownloader.figshare.com/files/65867442 https://ndownloader.figshare.com/files/65867439 https://ndownloader.figshare.com/files/65867457 https://ndownloader.figshare.com/files/65867445 https://ndownloader.figshare.com/files/65867448 https://ndownloader.figshare.com/files/65867454 https://ndownloader.figshare.com/files/65867451 https://ndownloader.figshare.com/files/65867460 https://ndownloader.figshare.com/files/65867463 Baumbach, Alexander Alexander Baumbach 0009-0005-0479-0091 Bioprocessing, bioproduction and bioproducts Industrial microbiology (incl. biofeedstocks) Halomonas 2026-06-26 2026-06-26 Dataset 2026 University College London Microbial growth phenotype is commonly summarised by a small set of parameters: specific growth rate, lag phase duration, maximum cell density, and growth curve profile; yet even within a single genus these characteristics can vary substantially across species, with direct consequences for how growth performance is interpreted and compared. This challenge is particularly pertinent for the largely uncharacterised Halomonas genus, whose members are increasingly proposed as next-generation industrial biotechnology chassis owing to their halotolerance and capacity for open, non-sterile cultivation. In this work, the growth phenotypes of 82 Halomonas strains were assessed under collection-recommended conditions of temperature, growth medium, and salt concentration using optical density measurements in 96-well plates. Maximum specific growth rates ranged from 0.05 ± 0.03 to 1.14 ± 0.34 h⁻¹ (mean 0.44 ± 0.22 h⁻¹), with the shape of the growth curve frequently deviating from the canonical sigmoidal form and displaying substantial inter-strain variability. No meaningful correlation was identified between any growth parameter and either cultivation temperature or salt concentration (−0.39 ≤ ρ ≤ 0.39). Considered collectively, growth-associated parameters identified H. hamiltonii, H. zhaodongensis, H. heilongjiangensis, and H. magadiensis as promising candidates for future biomanufacturing applications. The variability observed in growth curve profiles raised a critical methodological concern regarding researcher-dependent identification of growth phases and the computation of growth parameters. Six independent analysts assessed the same dataset, and at least one determined a significantly different maximum specific growth rate for half of the strains examined (p < 0.05). This has broad implications beyond strain characterisation: analyst interpretation directly affects reported growth rates, which serve as key inputs and constraints in metabolic modelling and upstream bioprocess design. The reliability of both primary literature and secondary computational analyses built upon these data is therefore directly affected by analyst-specific decisions, a problem further compounded for poorly characterised organisms, whose growth parameters are often borrowed from related species in the absence of species-specific data, introducing a largely unacknowledged source of uncertainty. This foundational phenotypic dataset highlights an urgent need for standardised, generalisable frameworks for growth parameter determination, particularly for non-model organisms.

]]>