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Identification, characterisation, rational design and mechanistic study of antimicrobial peptides from <i>Kassinasenegalensis</i>, the Senegal running frog Yuhai Cai (24168117) PUREID: 527955617 antimicrobial peptides rational design The rising global problem of antibiotic resistance, coupled with the slow development of newdrugs, has intensified the search for innovative antimicrobials. In this context, antimicrobialpeptides (AMPs) from the skin secretions of amphibians have been proposed due to theirunique antibacterial mechanism, strong antibacterial activity and low probability of drugresistance. In Chapter 3, this study successfully identifies the hitherto uncharacterized peptideQUB-1770 (FLPFLIPVISSLIKAL-NH2) from the skin secretions of the Senegal running frog(Kassina senegalensis). Especially against Staphylococcus aureus and MRSA, QUB-1770showed strong antibacterial effect against Gram-positive bacteria. It has a stronganti-proliferation effect on human H838 cancer cells. In Chapter 4, the structure-functionrelationship of the natural peptide QUB-1770 was studied to optimize its biological activity. 11analogues were designed using QUB-1770 as a template, and the effects of imperfectamphipathic structure and perfect amphipathic structure on biological activity were evaluated.Some of the designed analogues showed effective antibacterial activity. QUB-1770 and leadAMPs were selected to study the structure-activity relationship. In Chapter 5, a group ofpeptides inspired by Kassinakinin S was created and synthesized to explore their biologicalactivity and to discover effective bioactive peptides to prevent the spread of antibioticresistance. From the discovery and evaluation of novel AMPs in frog skin secretions to thedevelopment of AMPs with improved therapeutic indicators, this project has fully demonstratedthe possibility of finding natural AMPs from amphibian skin secretions, as well as the possibilityof making these available in the clinic through design and modification.<br><br><i>Thesis embargoed until 31st December 2028</i> 2026-10-01T16:46:01Z Text Thesis 10.17034/32638551.v1 https://figshare.com/articles/thesis/Identification_characterisation_rational_design_and_mechanistic_study_of_antimicrobial_peptides_from_i_Kassinasenegalensis_i_the_Senegal_running_frog/32638551 All Rights Reserved Open Access after 2028-12-31