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oai:figshare.com:article/32638209 2026-10-01T16:46:51Z portal_1172 item_type_8 month_year_10_2026

Identification and activity assessment of a bioactive peptide, QUB-2013, from the skin secretion of the Broad-folded Frog, (Phyllomedusa hypochondrialis) Haoyu Gan (24168018) PUREID: 526185828 Phylloseptin Antimicrobial peptides frog skin secretions molecular cloning antiproliferative effects A complex array of antimicrobial peptides, neuroactive peptides, peptidase inhibitors, etc, representing a collection of physiologically- and structurally-interacting components, are found in frog skin secretions. Phylloseptins are a class of peptides produced from frog skin with an active structural sequence that displays antmicrobial efficacy in vitro against the E.coli, S.aureus and C.albicans.<br><br>In the current study, a peptide (FLSLIPTAINAVSALAKHF-NH2), named QUB-2013, was discovered in Phyllomedusa hypochondrialis skin secretion by use of molecular cloning, and the crude peptide was subsequently obtained by solid phase synthesis and purified by reverse phase HPLC. MALDI-TOF MS was used to confirm the molecular mass, and a number of bioassays were performed on QUB-2013. As a result, QUB- 2013 was found to have anticancer cell activity and no appreciable cytotoxicity to horse red blood cells at concentrations less than 512 µM. Meanwhile, QUB- 2013 displayed antibacterial activity against Candida albicans below 256 µM and against E. coli and S. aureus below 128 µM. Furthermore, QUB-2013, in an MTT assay, was active at a concentration of 200 µM against NCI-H838 cancer .<br><br><i>Thesis is embargoed until 31 December 2028.</i> 2026-10-01T16:46:51Z Text Thesis 10.17034/32638209.v1 https://figshare.com/articles/thesis/Identification_and_activity_assessment_of_a_bioactive_peptide_QUB-2013_from_the_skin_secretion_of_the_Broad-folded_Frog_Phyllomedusa_hypochondrialis_/32638209 All Rights Reserved Open Access after 2028-12-31