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oai:figshare.com:article/32633343 2026-10-01T17:04:06Z portal_1172 item_type_8 month_year_10_2026

The discovery, identification, and bioactivity screening of peptide, QUB-2335, from skin secretion of the frog, Amolops hainanensis Wenjia Zhao (4898182) PUREID: 365374188 Antimicrobial peptides (AMPs) antibiotic resistance Hainanenin amphibian skin secretion QUB-2335 Due to the resistance of antibiotics, the research of antimicrobial peptides has become a hot topic. In this study, a novel peptide named, QUB-2335, was identified and characterised from the skin secretion of the frog (Amolops hainanensis) by the strategy of ‘shotgun’ cloning. The deduced mature peptide sequence was confirmed as: FALGAVTKRLPSLFCLITRKC. QUB-2335 showed a broad-spectrum antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and yeast. The result of QUB-2335 towards E. coli and S. aureus in MIC assays, were 4 μM and 4 μM, and in MBC assays, were 16 μM and 8 μM. Also, the MIC/MBC values of QUB-2335 against C. albicans, were is 64 μM and 128 μM. The haemolysis assay results showed that the QUB-2335 had only 27.1% haemolysis at the concentration of 256μM. The anticancer cell assay revealed a potent inhibition of cancer cell proliferation. At a concentration of 10-4 M, a significant impact of QUB-2335 was observed. These experiments on QUB-2335 revealed antibacterial and anticancer cell activities with potential of low side effects. Thus, this peptide has some value and potential to become a clinical drug after further research and modification.<br><br><i>Thesis embargoed until 31st December 2027 </i> 2026-10-01T17:04:06Z Text Thesis 10.17034/32633343.v1 https://figshare.com/articles/thesis/The_discovery_identification_and_bioactivity_screening_of_peptide_QUB-2335_from_skin_secretion_of_the_frog_Amolops_hainanensis/32633343 All Rights Reserved Open Access after 2027-12-31