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oai:figshare.com:article/32631825 2026-10-01T17:16:27Z portal_1172 item_type_8 month_year_10_2026

Identification and functional study of a novel bioactive peptide QUB-1761 from the venom of the Brazilian yellow scorpion, Tityus serrulatus Xinyue Wang (286629) PUREID: 250509228 Scorpion venom non-disulphide bridged peptide Gram-positive and Gram-negative bacteria human lung cancer haemolysis activity Scorpion venom contains a variety of peptides exerting broad bioactivities. In this thesis, QUB-1761, a non-disulphide bridged peptide (NDBP), was discovered in the venom of the Brazilian yellow scorpion, Tityus serrulatus (T. serrulatus). The peptide was identified through molecular cloning by using a sense primer. The synthetic peptide was obtained by solid phase peptide synthesis (SPPS), followed by purification and confirmation through reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). In broth microdilution assays, QUB-1761 had strong inhibitory effects on both Gram-positive and Gram-negative bacteria (MIC=8 µM for Staphylococcus aureus, MIC=32 µM for Escherichia coli). However, it was of low potency against yeast (MIC=128 µM for Candida albicans). In MTT anticancer cell proliferation and trypan blue exclusion assays, QUB-1761 beyond the concentration of 40 µM can completely kill the human lung cancer cells (NCI-H838). In terms of the haemolytic assays, QUB-1761 showed about 30% haemolysis activity at 64 µM and more than 50% at 128 µM. Nonetheless, QUB-1761 has the potential to be used in the design of drugs to treat bacterial infections or cancer if optimised.<br><br><i>Thesis embargoed until 31 December 2026</i>. 2026-10-01T17:16:27Z Text Thesis 10.17034/32631825.v1 https://figshare.com/articles/thesis/Identification_and_functional_study_of_a_novel_bioactive_peptide_QUB-1761_from_the_venom_of_the_Brazilian_yellow_scorpion_Tityus_serrulatus/32631825 All Rights Reserved Open Access after 2026-12-31