2026-10-14T14:37:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32631807 2026-10-01T17:16:37Z portal_1172 item_type_8 month_year_10_2026

Discovery and bioactivity assessment of a novel peptide from the skin secretion of the Senegal running frog Kassina senegalens Chunyuan Zhou (7543340) PUREID: 250506595 Antimicrobial peptide bioactivity assessment broad-spectrum antimicrobial activity low haemolysis activity novel peptide Frog skin is characterised with an abundance of biologically active antimicrobial peptides (AMPs) with a broad-spectrum effect against a variety of microorganisms. The contribution of their complex and diverse structures, physicochemical properties and mechanisms of biological activity, have been discovered and widely reported. In this study, a novel peptide named QUB-1923 was identified from the skin secretion of the Senegal running frog, Kassina senegalensis. The sequence of the mature peptide (FYHLSALIHGIKQRLK) was deduced from the cloned cDNA encoding a prepropeptide. Subsequently, this peptide was chemically synthesised by solid-phase synthesis. Following on from the purification by RP-HPLC and MALDI-TOF MS, the antimicrobial, anti-cancer cell proliferative and haemolytic activities of the purified peptide QUB-1923 were investigated. Results from antimicrobial assays have shown that this peptide has a broad-spectrum antimicrobial effect on S. aureus, E. coli and C. albicans. Notably, this peptide displayed the highest antimicrobial activity against E. coli (MIC=4 μM, and MBC=8 μM). On the other hand, this peptide only caused approximately 20% growth inhibition on lung cancer cell line H838 at a concentration of 100 μM. Additionally, this peptide demonstrated less than 10% haemolysis at a concentration of 256µM. These results suggest that this novel peptide is worthy of attention as a new antibacterial lead due to its potent inhibitory and bactericidal effects against Gram-ve bacteria, along with a low level of haemolysis.<br><i><br>Thesis embargoed until 31 December 2026</i>. 2026-10-01T17:16:37Z Text Thesis 10.17034/32631807.v1 https://figshare.com/articles/thesis/Discovery_and_bioactivity_assessment_of_a_novel_peptide_from_the_skin_secretion_of_the_Senegal_running_frog_Kassina_senegalens/32631807 All Rights Reserved Open Access after 2026-12-31