2026-10-14T16:57:29Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32628180 2026-10-01T17:41:38Z portal_1172 item_type_8 month_year_10_2026

Characterized analysis of peptide QUB-1433 from the skin secretion of the dark-spotted frog (Pelophylax nigromaculatus) Wenjie Wang (413433) PUREID: 183470520 Bioactive peptide isolated from the granular glands of frog skin have been widely discovered to exhibit antimicrobial and anticancer activities. Since the antibiotic resistance has become a severe difficulty in modern therapeutic treatment, many of them have been developed into clinical application for the treatment of microbial infection and cancer.<br><br>In this thesis, a potent natural antimicrobial peptide QUB-1433 was identified and cloned from the skin secretion of Pelophylax nigromaculatus via “shotgun” cloning. According to the amino acid residues of QUB-1433, the pure peptide was synthesised and purified via solid-phase peptide synthesis and RP-HPLC. Subsequently, antimicrobial, anticancer and haemolysis assays were performed on QUB-1433 for its functional evaluation. From the results of a series of bioactive assays, QUB-1433 displayed activities of against Gram-positive bacteria and fungi (MIC values are 16 and 32 µM), but is lack in broad-spectrum of anticancer ability (MIC value is 128 µM). Moreover, QUB-1433 possesses relative low cytotoxicity towards mammalian cell. Hence the bioactivities of QUB-1433 provided new insight in the discovery of new drugs. However, the mechanisms of action and optimization of QUB-1433 still required the further exploration. <br><br><i>Thesis embargoed until 31st October 2024 </i> 2026-10-01T17:41:38Z Text Thesis 10.17034/32628180.v1 https://figshare.com/articles/thesis/Characterized_analysis_of_peptide_QUB-1433_from_the_skin_secretion_of_the_dark-spotted_frog_Pelophylax_nigromaculatus_/32628180 All Rights Reserved