2026-10-14T07:01:47Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32628177 2026-10-01T17:41:40Z portal_1172 item_type_8 month_year_10_2026

QUB-1475: A novel bioactive peptide from the skin secretion of frog Hylarana Guentheri Jiewen Zheng (5684273) PUREID: 183470393 As antimicrobial resistance has become a threat towards human health for decades, the demand for the development of an alternative, broad-spectrum antimicrobial agent has turned into increasingly essential. As a consequence, the emergence of the antimicrobial peptide (AMP) derived from the skin secretion of amphibians has promoted the development of novel potential clinical therapeutics. Therefore, a novel potential bioactive peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied. <br>In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri. This thesis aimed to isolate and characterise the structure of the novel peptide QUB-1475 through “shotgun” molecular cloning, reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) analysis. Then the peptide was chemically-synthesised and evaluated through a series of bioactivity assays.<br>This peptide displayed no antimicrobial activity on S. aureus, E. coli and C. albicans conducted in this research. The IC50 values of the peptide towards NCI-H23 cell line, PC3 cell line and U-251 MG cell line are 26.51 μM, 10.03 μM, and 44.78 μM respectively. Moreover, it should be emphasised that the novel peptide presented haemolytic rate of 18.95% at the concentration of 512 μM. <br><br><i>Thesis embargoed until 30th September 2024 </i> 2026-10-01T17:41:40Z Text Thesis 10.17034/32628177.v1 https://figshare.com/articles/thesis/QUB-1475_A_novel_bioactive_peptide_from_the_skin_secretion_of_frog_Hylarana_Guentheri/32628177 All Rights Reserved