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oai:figshare.com:article/32626953 2026-10-01T17:46:24Z portal_1172 item_type_8 month_year_10_2026

The discovery and bioactivity evaluation of a novel peptide, QUB-2652, from the skin secretion of the South American frog, Phyllomedusa palliata Ziyi Dong (8938655) PUREID: 157905129 In recent years, the antibiotic resistance of bacteria has stimulated the development of new antibiotics. Antimicrobial peptides (AMPs) from amphibian skin secretions possess broad-spectrum antimicrobial activities through the mechanism that is unlikely to induce antibiotic resistance. Thus, AMPs are great candidates for new antibiotics. <br>In this study, the precursor-encoding cDNA sequence of QUB-2652 was identified in the skin secretion of Phyllomedusa palliata by ‘Shotgun’ cloning. Then, QUB-2652 was synthesised by solid phase peptide synthesis (SPPS) according to its translated putative mature peptide sequence. After authenticated by Matrix-assisted laser desorption/ionisation Time-of-flight mass spectrometry and purified by Reversed-phase high performance liquid chromatography, the pure QUB-2652 was finally subjected to some functional tests. <br>According to the BLAST analysis, QUB-2652 showed high sequence similarity with dermaseptins. Results of functional tests showed that QUB-2652 possessed prominent antimicrobial activities against E. coli, S. aureus and C. albicans with the minimum inhibitory concentrations of 2, 2 and 1 µM, respectively. QUB-2652 also showed strong anti-proliferative effects on NCI-H157, PC-3 and U251MG cell lines. Meanwhile, the haemolytic activity of QUB-2652 on horse erythrocytes was 3.6% at 1 µM and 10.6% at 2 µM. <br>This study is expected to inspire further studies on peptides from frog skin secretions and provide useful clues for developing new peptide antibiotics and anticancer drugs. 2026-10-01T17:46:24Z Text Thesis 10.17034/32626953.v1 https://figshare.com/articles/thesis/The_discovery_and_bioactivity_evaluation_of_a_novel_peptide_QUB-2652_from_the_skin_secretion_of_the_South_American_frog_Phyllomedusa_palliata/32626953 All Rights Reserved