2026-10-15T07:35:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32578317 2026-06-04T14:40:13Z category_24781 category_24790 category_24799 category_24802 category_24778 portal_753 item_type_3 month_year_06_2026

Analysis of the expression levels of prostate-specific antigen (PSA) and associated biomarkers in response to docetaxel (DTX) treatment using LNCaP and DU145 PCa cell lines Egnesious Sambo (24124191) Cancer diagnosis Chemotherapy Molecular targets Predictive and prognostic markers Cancer cell biology Docetaxel Prostate-specific antigen (PSA) Androgen receptor translocation p53 complex Cyclin-dependent kinase 2 (CDK2) activity p27 signaling SDG 3 Good health and wellbeing <p dir="ltr">The study aimed to determine the expression levels of PSA and associated biomarkers in response to DTX treatment using LNCaP and DU-145 PCa cell lines. Methodologically, the KEGG network and Reactome repository were used to map molecular checkpoints interlinked to the androgen receptor (AR) pathway under DTX exposure. Furthermore, six key regulatory checkpoints were selected for blind molecular docking with DTX using CB-Dock2 software to evaluate their respective binding affinities. Thereafter, the Crystal Violet cell viability assays were conducted to determine cytotoxicity, while the enzyme-immunosorbent assays (ELISAs) were utilised to quantify the free PSA (fPSA) and total PSA (tPSA) expression levels relative to the cytotoxic effects of DTX.</p> 2026-06-04T14:40:13Z Dataset Dataset 10.25403/UPresearchdata.32578317.v1 https://figshare.com/articles/dataset/Analysis_of_the_expression_levels_of_prostate-specific_antigen_PSA_and_associated_biomarkers_in_response_to_docetaxel_DTX_treatment_using_LNCaP_and_DU145_PCa_cell_lines/32578317 CC BY 4.0