2026-10-07T09:47:19Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32334711 2026-09-23T07:44:33Z category_24439 item_type_3 month_year_09_2026

<b>Mito-Nuclear Discordance and Fine-Scale Genomic Structuring in the Desert Lizard</b> haifan YAN (23892546) Vertebrate biology Phrynocephalus axillaris Tarim Basin phylogeography population genetic structure mito-nuclear discordance tectonic uplift aridification <p dir="ltr">This dataset contains all original molecular data supporting the research article entitled "Mito-Nuclear Discordance and Phylogeographic Structuring in the Desert Lizard <i>Phrynocephalus axillaris</i> Driven by Pleistocene Oscillations". Tail tissue samples were collected from 239 <i>P. axillaris</i> individuals at 21 sampling localities across the Tarim Basin and adjacent regions in Xinjiang Uygur Autonomous Region, China. Complete sequences of four mitochondrial genes (ATP6, COI, Cytb, ND4) and three nuclear loci (RAG1, AKAP9, BDNF) were obtained via Sanger sequencing.</p><p dir="ltr">This study sampled 239 individuals of <i>Phrynocephalus axillaris</i> from 21 geographic populations across the Tarim Basin and surrounding areas in Xinjiang, China. Tail tip tissues of approximately 5 mm² were collected and stored at 4 °C in a 1:1 (v/v) mixture of sterile normal saline and absolute ethanol. Genomic DNA was extracted and amplified via PCR. Sanger sequencing yielded full-length sequences of four mitochondrial genes (ATP6, COI, Cytb, ND4) and three nuclear genes (RAG1, AKAP9, BDNF). Multiple software programs including Geneious, MAFFT, SequenceMatrix, DnaSP, Network, PartitionFinder, RAxML, MrBayes and BEAST were applied for sequence assembly, alignment, concatenation, as well as analyses of genetic diversity, haplotype network, phylogeny and divergence time.</p> 2026-09-23T07:44:33Z Dataset Dataset 10.6084/m9.figshare.32334711.v3 https://figshare.com/articles/dataset/_b_Mito-Nuclear_Discordance_and_Fine-Scale_Genomic_Structuring_in_the_Desert_Lizard_b_/32334711 CC BY 4.0