2026-10-14T13:13:10Z https://api.figshare.com/v2/oai

oai:figshare.com:article/32251290 2026-10-02T12:18:48Z portal_1132 item_type_3 month_year_10_2026

Parallelised detection of bacterial viability using an electrode array and the Exeter Multiscope - dataset Ka Kiu Lee (21040091) David Horsell (21058118) Tailise de Souza Guerreiro Rodrigues (21051857) Stefano Pagliara (21068567) Alexander Corbett (21068960) antimicrobial susceptibility testing (AST) <p dir="ltr">This is the supplemental data for <b>Parallelised detection of bacteria viability using an electrode array and the Exeter Multiscope</b>, published in Scientific Reports, volume 16, article 29903. https://doi.org/10.1038/s41598-026-66381-9</p><p dir="ltr">The research is summarised below:</p><p dir="ltr">Antimicrobial resistance remains a global existential threat. Given that antimicrobial therapy commonly starts before pathogen identification, rapid and scalable methods capable of determining effective antimicrobial compounds are needed. In this paper, we demonstrate a 2 × 2 array of parallelised microscopes that uses low numerical aperture (NA=0.25) detection optics and LED excitation to determine bacterial viability based on their fluorescence response to an electrical stimulus. Following a 2-hour incubation, the fluorescent viability readout requires less than one minute. We use K-means clustering to classify pixels in a time lapse sequence of widefield fluorescence images and extract changes seen within bacterial clusters. We demonstrate sufficient sensitivity to measure fluorescence changes after electrical stimulation in a bacterial monolayer. To capture these subtle fluorescence changes at high signal-to-background ratios, we place a limit on the minimum optical density of the bacterial sample. This novel approach is scalable to 96-well formats using a suitable consumable electrode array.</p> 2026-05-13T00:00:00Z Dataset Dataset 10.24378/exe.32251290.v1 https://figshare.com/articles/dataset/Parallelised_detection_of_bacterial_viability_using_an_electrode_array_and_the_Exeter_Multiscope_-_dataset/32251290 CC BY