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        <datestamp>2026-09-27T06:50:37Z</datestamp>
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          <dc:title>&lt;b&gt;Genome assembly and annotation of &lt;/b&gt;&lt;b&gt;&lt;i&gt;Verticillium alfalfae&lt;/i&gt;&lt;/b&gt;&lt;b&gt; strain Va8&lt;/b&gt;</dc:title>
          <dc:creator>luran Wang (23859462)</dc:creator>
          <dc:subject>Genomics</dc:subject>
          <dc:subject>Mycology</dc:subject>
          <dc:subject>Verticillium alfalfae</dc:subject>
          <dc:subject>alfalfa wilt</dc:subject>
          <dc:subject>genome assembly</dc:subject>
          <dc:subject>Va8</dc:subject>
          <dc:subject>fungal pathogen</dc:subject>
          <dc:description>&lt;pre&gt;&lt;p dir="ltr"&gt;&lt;i&gt;Verticillium alfalfae&lt;/i&gt; is the causal agent of alfalfa Verticillium wilt, a destructive disease that causes significant economic losses. To elucidate the genetic basis of its pathogenicity, we sequenced the genome of &lt;i&gt;V. alfalfae&lt;/i&gt; strain Va8 using the PacBio Sequel single-molecule real-time (SMRT) sequencing platform, supplemented with Illumina short-read sequencing for error correction. Genome assembly was performed using Hifiasm, yielding a high-quality genome assembly at the contig level. The assembled genome is 33,951,091 base pairs (bp) in size and comprises 8 contigs, with a contig N50 of 3,990,853 bp and a contig N90 of 3,057,321 bp. A total of 771,686 bp of repetitive sequences and 9,441 protein-coding genes were predicted, of which 9,425 were functionally annotated using multiple public databases. This study enhances our understanding of the genetic basis of &lt;i&gt;V. alfalfae&lt;/i&gt; pathogenicity and provides a foundation for future research on disease control and fungal evolution.&lt;/p&gt;&lt;/pre&gt;&lt;p&gt;&lt;/p&gt;</dc:description>
          <dc:date>2026-09-27T06:50:37Z</dc:date>
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          <dc:identifier>10.6084/m9.figshare.32226969.v5</dc:identifier>
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          <dc:rights>CC BY 4.0</dc:rights>
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