2026-10-06T17:21:09Z https://api.figshare.com/v2/oai

oai:figshare.com:article/31947231 2026-09-15T05:36:26Z category_24436 category_24244 item_type_3 month_year_09_2026

A megabarcoding insect reference library highlighting immature stages and dark taxa from contrasting temperate and subtropical ecosystems in China wenya Pei (18103261) Invertebrate biology Animal systematics and taxonomy megabarcoding, dark taxa, biodiversity, malaise trap <p dir="ltr">We present the Megabarcoding Insect Reference: Tianjin and Guangxi (MIR-TG), a comprehensively vouchered DNA barcode library addressing two persistent bottlenecks in insect biodiversity science: the identification of immature life stages and the documentation of hyperdiverse “dark taxa”. Sampling across contrasting temperate and subtropical ecosystems in China, we employed a highly scalable MinION-based megabarcoding workflow. To maximize data recovery across varying DNA preservation states, a cost-effective HotSHOT extraction was coupled with a dual-amplicon strategy targeting both full-length (658-bp) and mini-barcode (313-bp) <i>COI</i> fragments. From 2,332 individual specimens, we generated 2,174 high-quality sequences. This pipeline yielded a consolidated reference of 593 morphologically defined units across 9 orders and 134 families.</p> 2026-09-15T05:36:26Z Dataset Dataset 10.6084/m9.figshare.31947231.v1 https://figshare.com/articles/dataset/A_megabarcoding_insect_reference_library_highlighting_immature_stages_and_dark_taxa_from_contrasting_temperate_and_subtropical_ecosystems_in_China/31947231 CC BY 4.0