2026-10-11T20:40:28Z https://api.figshare.com/v2/oai

oai:figshare.com:article/31832125 2026-09-18T03:18:11Z category_24295 item_type_3 month_year_09_2026

Integration of scRNA-seq and scATAC-seq revealed the role of lncRNA <i>RP11-661C8.2</i> by promoting EMT in clear cell renal cell carcinoma Zhenyuan Yu (6698111) Epigenetics (incl. genome methylation and epigenomics) scRNA-seq and snATAC-seq integration <p dir="ltr">Long noncoding RNAs (lncRNAs) play an important role in tumor progression. However, most of the previously discovered lncRNAs that promote or inhibit tumor progression were derived from bulk RNA-seq results. Data from single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq) in clear cell renal cell carcinoma (ccRCC) were integrated to precisely identify the lncRNAs that regulate tumor progression in ccRCC. The scRNA-seq data of 23 ccRCC samples and the scATAC-seq data of 19 samples were analysed, capturing the transcriptome of 121,106 cells an<a href="" target="_blank">d the </a>epigenetic features of 61,693 cells, respectively. Results showed that lncRNA <i>RP11-661C8.2</i> was associated with significant chromatin accessibility in ccRCC tumor cells by scATAC-seq. Subsequently, integrated scRNA-seq analysis and functional experiments <i>in vitro and in vivo</i> confirmed the role of <i>RP11-661C8.2</i> in promoting the invasion, migration and <a href="" target="_blank">epithelial-mesenchymal transition</a> (EMT) of ccRCC cells. In addition, <i>RP11-661C8.2</i> played a role in up-regulating <i>ITGAV</i> and activating the PI3K/AKT signaling pathway, which may be the potential mechanism of promoting the EMT in ccRCC cells. This study identified the role of<i> RP11-661C8.2</i> in ccRCC, which provided new reference basis and potential intervention targets for ccRCC.</p> 2026-09-18T03:18:11Z Dataset Dataset 10.6084/m9.figshare.31832125.v1 https://figshare.com/articles/dataset/Integration_of_scRNA-seq_and_scATAC-seq_revealed_the_role_of_lncRNA_i_RP11-661C8_2_i_by_promoting_EMT_in_clear_cell_renal_cell_carcinoma/31832125 CC BY 4.0