2026-10-06T12:47:55Z https://api.figshare.com/v2/oai

oai:figshare.com:article/31455097 2026-09-28T15:47:20Z category_26269 category_24343 item_type_3 month_year_09_2026

Machine-learning guided discovery of emergent antimicrobial activity from dynamic covalent assemblies（2b-RAD-M） Yuanfeng Li (21088505) Ji-yang Chen (22490594) Yinzi Piao (21088633) Zhanpei Bai (23291914) haoyue wu (22105418) Yu-han Zhao (23291972) Yuqin Wang (23291993) Haibo Zhang (5061767) Xiufeng Huang (2887607) Tieli Zhou (129625) Jian Li (41607) Linqi Shi (21088642) Yong Liu (19677156) Biomaterials Nanobiotechnology machine-learning functions nanobiotechnology platform <p dir="ltr">Species-level oral microbiota profiling was performed using 2bRAD-M sequencing in a Streptococcus mutans-induced rat caries model. This dataset includes 24 biological samples: six healthy controls, six PBS-treated caries controls, six A5B5-treated samples and six chlorhexidine (CHX)-treated samples. Abundance.filtered.anno.csv contains all 24 samples in one annotated species-by-sample relative-abundance matrix. sample_metadata.csv provides the corresponding sample groups and original sample identifiers.</p><p dir="ltr"><br></p><p dir="ltr">Methods documented for the original samples: Library preparation and sequencing were conducted by OE Biotech (Qingdao, China) using a Type IIB restriction enzyme-based 2bRAD-M workflow, including BcgI digestion, adaptor ligation, PCR amplification, barcode indexing, and sequencing on the Illumina NovaSeq PE150 platform. For downstream analysis, quality-controlled 2bRAD tags were mapped to a species-resolved 2bRAD marker database constructed from GTDB and Ensembl microbial genomes; candidate taxa were filtered using a Gscore threshold (>5) to reduce false positives, and species-level relative abundances were computed based on mapped reads and species-specific marker counts according to published 2bRAD-M protocols.</p> 2026-03-04T05:59:22Z Dataset Dataset 10.6084/m9.figshare.31455097.v2 https://figshare.com/articles/dataset/Machine-learning_guided_discovery_of_emergent_antimicrobial_activity_from_dynamic_covalent_assemblies_2b-RAD-M_/31455097 CC BY 4.0