2026-10-06T06:18:20Z https://api.figshare.com/v2/oai

oai:figshare.com:article/31451563 2026-09-28T15:47:10Z category_26269 category_24343 item_type_3 month_year_09_2026

Machine-learning guided discovery of emergent antimicrobial activity from dynamic covalent assemblies (<i>Streptococcus mutans</i>, RNA seq) Yuanfeng Li (21088505) Ji-yang Chen (22490594) Yinzi Piao (21088633) Zhanpei Bai (23291914) haoyue wu (22105418) Yuhan Zhao (337301) Yuqin Wang (23291993) Haibo Zhang (5061767) Xiufeng Huang (2887607) Tieli Zhou (129625) Jian Li (41607) Linqi Shi (21088642) Yong Liu (19677156) Biomaterials Nanobiotechnology machine-learning functions Biotechnology platform <p dir="ltr"><i>This dataset contains bulk RNA-seq data from Streptococcus mutans under three conditions: PBS control, A5B5 treatment for 0.5 h at a sub-MIC concentration, and A5B5 treatment for 4 h, with three biological replicates per condition (nine samples in total). counts_anno.csv and FPKM_anno.csv each contain all nine samples in a single annotated gene-expression matrix. The matrices include 1,850 gene identifiers present in both source datasets. CTL-1/2/3 are PBS controls, A5B5-1/2/3 are the 4-h samples, and A5B5_0.5h-1/2/3 are the 0.5-h samples. The sample groups and original identifiers are provided in sample_metadata.csv.</i></p><p dir="ltr"><br></p><p dir="ltr">Methods documented for the original samples: Total RNA was extracted using the RNAprep Pure Cell/Bacteria Kit (TIANGEN, Beijing, China; Cat. No. DP430) according to the manufacturer’s protocol. RNA purity and concentration were measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), and RNA integrity was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Only RNA samples with qualified purity, quantity, and integrity were used for library preparation. Ribosomal RNA was removed using the TIANSeq rRNA Depletion Kit (TIANGEN, Beijing, China). Strand-compatible transcriptome libraries were then prepared using the VAHTS Universal V6 RNA-seq Library Prep Kit following the manufacturer’s instructions. Library sequencing was performed by OE Biotech Co., Ltd. (Shanghai, China).</p> 2026-03-04T06:01:55Z Dataset Dataset 10.6084/m9.figshare.31451563.v2 https://figshare.com/articles/dataset/Machine-learning_guided_discovery_of_emergent_antimicrobial_activity_from_dynamic_covalent_assemblies_i_Streptococcus_mutans_i_RNA_seq_/31451563 CC BY 4.0