2026-10-06T19:35:02Z https://api.figshare.com/v2/oai

oai:figshare.com:article/31294000 2026-09-28T09:20:09Z category_24295 category_24784 item_type_3 month_year_09_2026

Single-cell multiome data of 4 LOY clones and 4 ROY clones of A549 cell lines Michael Scherer (12212429) Kathleen Schlüter (23156971) Mei Ju Chen (23156976) Maria Llamazares-Prada (21632297) Christoph Plass (14946945) Epigenetics (incl. genome methylation and epigenomics) Cancer genetics 10x multiome dataset epigenomics and epigenetics A549 mult-omics <p dir="ltr">Nuclei Isolation was performed according to 10X Genomics demonstrated protocol (Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing - CG000365) starting with 5x10⁵ fresh cells. Cells were incubated with the lysis buffer for 4 minutes. Single-nuclei ATAC and 3’ gene expression sequencing libraries were generated using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle (10X Genomics) according to the manufacturer’s instructions. Per well of the Chromium Next GEM Chip J (10X Genomics) one sample with 15,000 nuclei was loaded. Libraries were sequenced on a NovaSeq 6000 system using the S4 v1.5 flow cell with paired-end 200 cycles (1 lane for gene expression and 3 lanes for ATAC libraries). </p><p dir="ltr">Raw read data was processed using 10x Genomic’ Cellranger-ARC v2.0.2 software using default parameters to generate gene count matrices and fragment files for snRNA-seq and scATAC-seq, respectively.</p><p dir="ltr">The dataset contains the following layers:</p><p dir="ltr">RNA: scRNA-seq data of the 4 LOY and 4 ROY cell lines (metadata column: sample.name)</p><p dir="ltr">ATAC: scATAC-seq data for the same cells</p><p dir="ltr">Integration has been performed with Seurat's WNN method.</p> 2026-09-28T09:20:09Z Dataset Dataset 10.6084/m9.figshare.31294000.v1 https://figshare.com/articles/dataset/Single-cell_multiome_data_of_4_LOY_clones_and_4_ROY_clones_of_A549_cell_lines/31294000 CC BY 4.0