2026-10-07T23:53:59Z https://api.figshare.com/v2/oai

oai:figshare.com:article/31292941 2026-09-28T09:19:58Z category_24295 category_24784 item_type_3 month_year_09_2026

CyTOF analysis for A549 cell lines (4 LOY and 2 ROY) Michael Scherer (12212429) Kathleen Schlüter (23156971) Maria Llamazares-Prada (21632297) Mei Ju Chen (23156976) Christoph Plass (14946945) Epigenetics (incl. genome methylation and epigenomics) Cancer genetics CyTOF histone modifcation epigenomics and epigenetics <p dir="ltr">CyTOF data underwent the following pre-processing prior to analyses: First, the CyTOF software by Fluidigm was used for normalization and concatenation of the acquired data. Then, several gates were applied using the Cytobank platform (Beckman Coulter):</p><p dir="ltr">First, the normalization beads were gated out using the 140Ce channel. Then, live single cells were gated using the cisplatin 195Pt, iridium DNA label in 193Ir, event length, and the Gaussian parameters of width, center, offset and residual channels. CyTOF software was then used for samples de-barcoding.</p> 2026-09-28T09:19:58Z Dataset Dataset 10.6084/m9.figshare.31292941.v1 https://figshare.com/articles/dataset/CyTOF_analysis_for_A549_cell_lines_4_LOY_and_2_ROY_/31292941 CC BY 4.0