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oai:figshare.com:article/30537245 2026-09-29T07:37:36Z category_24094 item_type_3 month_year_09_2026

Antimicrobial peptide PMAP-36 attenuates ExPEC-induced neuroinflammation by inhibiting the NF-κB/NLRP3 pathway through autophagy activation Lianci Peng (22561466) Veterinary immunology PMAP-36 extraintestinal pathogenic E. coli anti-neuroinflammation autophagy activation Anti-inflammatory response Supplementary materialsFigure S1. The biocompatible effect of PMAP-36 in vitro and in vivo. (A) The viability of BV2 cells treated with PMAP-36 (0.625 μM or 1.25 μM) for 12 h, followed by washing and then incubation for an additional 24 h, was detected via the CCK-8 assay. Mice (total N=18) were intraperitoneally injected with 5 mg/kg PMAP-36 or PBS as a negative control. (B) Body weight was monitored daily for 7 d, and variations in body weights (%) (total N=12, per group n=6) are shown. (C-F) Blood was collected to determine biochemical indicators, including alanine aminotransferase (ALT) (C), aspartate aminotransferase (AST) (D), urea (UREA) (E) and inorganic phosphate (IP) (F). Different tissues (total N=6, per group n=3), including the brain, lung, liver, and spleen, were collected to observe histopathological changes. (G) Representative images of histopathological changes in tissues are shown. All data are presented as the means ± SEM from five, six, or three biological replicates. ns = not significant.Figure S2. The effect of PMAP-36 on bacterial invasion in BV2 cells. (A) The number of intracellular bacteria in ExPEC-infected BV2 cells with PMAP-36 treatment under the condition of 3-MA and CQ at 1 and 2 h post infection. Data are presented as the means ± SEM from three biological replicates. ***P ≤ 0.001, ns = not significant. 2025-11-05T03:39:57Z Dataset Dataset 10.6084/m9.figshare.30537245.v9 https://figshare.com/articles/dataset/_b_Antimicrobial_peptide_PMAP-36_attenuates_ExPEC-induced_neuroinflammation_by_inhibiting_the_NF-_b_b_b_b_B_NLRP3_pathway_through_autophagy_activation_b_/30537245 CC BY 4.0