2026-10-14T08:39:03Z https://api.figshare.com/v2/oai

oai:figshare.com:article/29565428 2026-10-01T07:03:33Z category_42 category_21 portal_81 item_type_3 month_year_10_2026

Reprogramming product selectivity and activity in promiscuous terpene synthases via substrate conformation engineering Sheng Dong (21717508) Hongshuang Liu (21717511) Weihong Liao (21717514) Xuedong Chen (21717517) Tianyu Cui (21717520) Ruimin Zhang (21717523) Ying Han (21717525) Lei Wang (21717526) Xiao Men (21717528) Haibo Zhang (21717531) Biological Engineering Biotechnology Terpene synthases Germacrene A synthase Product Selectivity enzyme engineering β-elemene This dataset contains gas chromatography (GC) data supporting the study on engineering the promiscuous terpene synthase FpGAS to reprogram its product selectivity and catalytic activity. Specifically, the data represent the product profiles of wild-type and various site-directed mutants of FpGAS, expressed in Escherichia coli, as determined by GC analysis. The dataset includes: Raw and processed GC peak data (retention time, peak area) for each mutant Calculated relative abundances (%) of major terpenoid products Product composition changes across different mutants These data illustrate the impact of substrate conformation modulation on the distribution of linear and cyclized terpenoid products, and support the mechanistic conclusions of the main manuscript. 2026-10-01T07:03:33Z Dataset Dataset 10.6084/m9.figshare.29565428.v1 https://figshare.com/articles/dataset/Reprogramming_product_selectivity_and_activity_in_promiscuous_terpene_synthases_via_substrate_conformation_engineering/29565428 CC BY 4.0