2026-10-08T06:31:12Z https://api.figshare.com/v2/oai

oai:figshare.com:article/28678313 2026-10-01T23:11:55Z category_24157 item_type_3 month_year_10_2026

Rab Interactions identified by DEEPN. Robert Piper (19005749) Christopher P. Ptak (4693714) Tabitha Peterson (24844092) Protein trafficking DEEPN Rab GTPase <p dir="ltr"><b>Rab GTPase Y2H Interactome — Verified Hit Dataset</b></p><p dir="ltr">This dataset contains the curated set of protein-protein interactions identified in a yeast two-hybrid (Y2H) screen against 36 human Rab GTPases, each tested in both nucleotide-locked conformations: GTP-bound (constitutively active, Q→L mutant) and GDP-bound (dominant-negative, T→N mutant). The dataset comprises 591 confirmed interactions, each supported by an Interaction Data Package (IDP) verification workbook containing 5' and 3' junction-mapping plots, sequencing read-depth coverage, and DESeq2/Bayesian statistical support (enrichment, log2 fold-change, and significance values) for both the selected and non-selected yeast populations.</p><p dir="ltr"><code>Unified_Rab_Interaction_List.xlsx</code> indexes all 591 hits with their RefSeq accession, estimated interacting fragment (amino acid span), and full statistics, hyperlinked to the corresponding IDP workbook. IDP files are organized into per-Rab, per-conformation subfolders (e.g. <code>Rab19-GTP</code>, <code>Rab19-GDP</code>). These data are also in Supplementary Table 3, which is an abridged version of these files.</p><p dir="ltr">Prior to inclusion, candidate interactions were filtered for biological plausibility: vector-alone (bait-independent) background artifacts, and interactions mapping to the extracellular/ER-lumenal domain of an integral membrane protein or to a secreted protein, were excluded as topologically inconsistent with a genuine cytosolic two-hybrid interaction. Excluded candidates and the rationale for each exclusion are provided in Supplementary Table 2.</p><p dir="ltr">Plasmids used are described in Supplementary Table 1. This calls out the figures the plasmids were used in. Maps of key plasmids are in the .zip file 'Plasmids_Used' in .gbk format as named in the Table.</p> 2026-10-01T23:11:55Z Dataset Dataset 10.6084/m9.figshare.28678313.v4 https://figshare.com/articles/dataset/Rab_Interactions_identified_by_DEEPN_/28678313 CC BY 4.0