2026-10-15T10:25:56Z https://api.figshare.com/v2/oai

oai:figshare.com:article/28654784 2026-06-03T21:11:13Z category_30187 portal_753 item_type_3 month_year_06_2026

Single molecule spectroscopy (SMS) analysis suite Joshua Botha (20927999) Biological physics Single molecule spectroscopy (SMS) Change-point analysis Light harvesting complexes Unbiased statistical analysis Time tagged single-photon detection Single-molecule biophysics <p dir="ltr">This study addressed challenges through the development of Full SMS, a versatile analysis software suite and a custom-designed sample cell holder, which together provide an integrated framework to advance SMS-based studies. Full SMS is a comprehensive graphical user interface (GUI)-driven software package for processing and analysing multi parameter SMS datasets. These datasets typically include fluorescence intensity and lifetime, as well as spectral properties of single dye molecules, quantum dots, or fluorescent active biomolecules. The software enables detailed analysis through tools for statistical evaluation of fluorescence intensity, clustering, and level identification; lifetime decay fitting; and second-order correlation function calculations. Full SMS is used to analyse three sample types as case studies to showcase its utility and ease-of-use. Complementing the software, this study also presents a custom-designed sample cell holder, which extends the capabilities of the SMS experimental setup designed and assembled in-house. This hardware innovation enhances stability and versatility, allowing for more robust and reproducible single molecule measurements under a wider range of experimental conditions. </p> 2026-06-03T21:11:13Z Dataset Dataset 10.25403/UPresearchdata.28654784.v1 https://figshare.com/articles/dataset/Single_molecule_spectroscopy_SMS_analysis_suite/28654784 CC BY 4.0