2026-06-18T15:39:22Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/27931947
2025-06-20T18:56:06Z
category_24223
portal_549
item_type_3
month_year_06_2025
10.5522/04/27931947.v1
https://figshare.com/articles/dataset/Meta16S_Custom_Reference_Database_Mexico_City_Fish_Herpetofauna/27931947
https://ndownloader.figshare.com/files/50885673
Maeda Obregon, Alejandro
Alejandro
Maeda Obregon
Day, Julia
Julia
Day
0000-0002-6765-7782
<![CDATA[Meta16S Custom Reference Database Mexico City Fish & Herpetofauna]]>
Freshwater ecology
Environmental DNA
2025-06-20
2025-06-20
Dataset
2025
University College London
FASTA file with the sequences for amphibian, fish and reptile species registered to occur in Mexico City, functioning as a custom reference database for the Meta16S metabarcoding library. Sanger sequences were obtained through DNA extractions from preserved tissues (obtained through the project's collaborators) using the QIAGEN DNeasy Blood & Tissue kit. The processing of the sequences (including de novo assembly) was done using Geneious Prime. Sequences were used during the Taxonomy Assignment step of the bioinformatic pipeline using Python packages BLAST+ and BASTA.
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