2026-06-13T03:41:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/27367908 2025-06-20T18:57:11Z category_24223 portal_549 item_type_3 month_year_06_2025

10.5522/04/27367908.v1 https://figshare.com/articles/dataset/Raw_reads_12S-V5_Illumina_MiSeq_metabarcoding_library/27367908 https://ndownloader.figshare.com/files/50099934 Maeda Obregon, Alejandro Alejandro Maeda Obregon Day, Julia Julia Day 0000-0002-6765-7782 Freshwater ecology Environmental DNA 2025-06-20 2025-06-20 Dataset 2025 University College London Demultiplexed raw reads of the 12S-V5 Illumina MiSeq metabarcoding library using the 2 X 150 chemistry kit. The metabarcoding contains sequences of ~110 bp from various vertebrate groups. The files contained include true samples, positive control and negative controls. The data contained in the files was assessed using the Python package FastQC, while processing and filtering were conducted using the R package dada2 for Amplicon Sequence Variants (ASVs) generation. Downstream analysis was performed using R package phyloseq and formatted for ecological analyses using additional R packages (e.g. vegan).

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