2026-08-08T12:29:07Z https://api.figshare.com/v2/oai

oai:figshare.com:article/27337557 2025-06-20T18:58:30Z category_24223 portal_549 item_type_3 month_year_06_2025

10.5522/04/27337557.v1 https://figshare.com/articles/dataset/Raw_reads_Meta16S_Illumina_MiSeq_metabarcoding_library/27337557 https://ndownloader.figshare.com/files/50083698 Maeda Obregon, Alejandro Alejandro Maeda Obregon Day, Julia Julia Day 0000-0002-6765-7782 Freshwater ecology Environmental DNA 2025-06-20 2025-06-20 Dataset 2025 University College London Demultiplexed raw reads of the Meta16S Illumina MiSeq metabarcoding library using the 2 X 250 chemistry kit. The metabarcoding contains sequences with a size range of 200 to 280 bp from both vertebrate and invertebrate groups. The files contained include true samples, positive control and negative controls. The data contained in the files was assessed using the Python package FastQC, while processing and filtering were conducted using the R package dada2 for Amplicon Sequence Variants (ASVs) generation. Downstream analysis was performed using R package phyloseq and formatted for ecological analyses using additional R packages (e.g. vegan).

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