2026-10-15T09:21:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/26403478 2024-07-31T16:04:03Z category_24136 category_24640 category_27199 category_24706 portal_753 item_type_3 month_year_07_2024

Evaluation of the antibacterial activity and mechanism of action of CPF-MW1 Kabelo Sitwe (17416521) Anabella Gaspar (12160418) Megan Bester (17889701) Analytical biochemistry Medical biochemistry - amino acids and metabolites Preventative health care Medical microbiology not elsewhere classified CPF-MW1 Antimicrobial peptide Membrane permeabilisation Antimicrobial resistance Multi-drug resistance Gram-negative bacteria Escherichia coli ATCC 700928 SDG 3 Good health and wellbeing SDG 6 Clean water and sanitation <p>The data provided represent the results of the titled study above. The study aimed to evaluate the antimicrobial activity and the mechanism of action of the frog-derived antimicrobial peptide CPF-MW1 against Gram-negative bacteria using E. coli ATCC 700928 as a model organism. The objectives of the study included the following:</p> <ol><li>Antibacterial activity determination (both anti-planktonic and anti-biofilm)</li><li>Peptide cytotoxicity evaluation both on human erythrocytes and HaCaT cell line.</li><li>Mechanism of action evaluation including time-kill kinetics, membrane permeabilisation and scanning electron microscopy.</li><li>Peptide stability in physiological conditions evaluated by pre-exposure to proteolytic enzyme (trypsin) and fetal calf serum.</li></ol> <p><br></p> <p>All data provided represents a set of three independent experiments performed in triplicate, unless stated otherwise. Data was analyzed and processed using GraphPad prism 8 software. Each data point was calculated as an average of three biological repeats each consisting of three technical repeats generating 9 data points unless stated otherwise. All data was expressed as means ± standard error of the means (SEM). The normality of data was first determined and depending on the normality of the data, multiple comparisons were tested using either the One-way ANOVA with Turkey’s post hoc tests, or the Kruskal-Wallis ANOVA with Duns’ post hoc tests. Significance was determined at a 95% confidence interval, and any p-value < 0.05 was identified as significant.</p> <p><br></p> 2024-07-31T16:04:03Z Dataset Dataset 10.25403/UPresearchdata.26403478.v1 https://figshare.com/articles/dataset/Evaluation_of_the_antibacterial_activity_and_mechanism_of_action_of_CPF-MW1/26403478 CC BY 4.0