2026-05-06T07:13:24Z https://api.figshare.com/v2/oai
oai:figshare.com:article/22193692 2023-03-20T16:18:59Z category_24508 category_24370 category_24634 portal_549 item_type_1 month_year_03_2023
10.5522/04/22193692.v1 https://figshare.com/articles/figure/3D_urine-tolerant_human_urothelial_model_to_study_urinary_tract_infection/22193692 https://ndownloader.figshare.com/files/39602557 https://ndownloader.figshare.com/files/39602656 https://ndownloader.figshare.com/files/39602662 https://ndownloader.figshare.com/files/39602689 https://ndownloader.figshare.com/files/39602701 https://ndownloader.figshare.com/files/39602806 https://ndownloader.figshare.com/files/39602827 https://ndownloader.figshare.com/files/39602890 https://ndownloader.figshare.com/files/39603163 https://ndownloader.figshare.com/files/39603166 https://ndownloader.figshare.com/files/39603220 https://ndownloader.figshare.com/files/39603271 https://ndownloader.figshare.com/files/39603301 https://ndownloader.figshare.com/files/39603319 https://ndownloader.figshare.com/files/39604675 https://ndownloader.figshare.com/files/39604741 10.3389/fcimb.2023.1128132 Jafari, Nazila Nazila Jafari Rohn, Jennifer Jennifer Rohn <![CDATA[3D urine-tolerant human urothelial model to study urinary tract infection]]> Infectious diseases Microbiology not elsewhere classified Immunology not elsewhere classified Cytokeratins Uroplakins tight junctions (TJs) adherence junctions 3D-UHU Infectious Diseases Microbiology Immunology 2023-03-20 2023-05-30 Figure 2023 University College London   

3D confocal images of:

Figure1: 3D-UHU model

Figure3: UPK1,II,III, and Chondroitin sulphate

Figure4: CK8, CK13, and CK20

Figure5: E-cadherin, ZO-1, Claudin1, and Claudin3

Figure6: TLR2, TLR4, and TLR5


3D-UHU grown on filter membranes were Immunostained then mounted with ProLong Glass antifade mountant (Invitrogen) and imaged on a Leica SP8 microscope. Images were acquired using 63x magnification with 0.3 µm Z step size giving 50% image overlap required for 3D image reconstruction. Images were processed using LAS X software version 3.5.7.

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