2026-06-01T16:39:51Z https://api.figshare.com/v2/oai
oai:figshare.com:article/19642395 2023-03-27T14:18:11Z category_24748 portal_549 item_type_3 month_year_03_2023
10.5522/04/19642395.v1 https://figshare.com/articles/dataset/Validation_of_mouse_model_qPCR_and_Western_blots_probing_for_Tor1a-torsinA/19642395 https://ndownloader.figshare.com/files/34887267 https://ndownloader.figshare.com/files/34887279 https://ndownloader.figshare.com/files/34887294 https://ndownloader.figshare.com/files/34887297 https://ndownloader.figshare.com/files/34887300 https://ndownloader.figshare.com/files/34887303 https://ndownloader.figshare.com/files/34887306 https://ndownloader.figshare.com/files/34887309 https://ndownloader.figshare.com/files/34887312 Pocratsky, Amanda Amanda Pocratsky Do Nascimento Xavier Fernandes, Filipe Filipe Do Nascimento Xavier Fernandes Ozyurt, Gorkem Gorkem Ozyurt White, Ian Ian White Sullivan, Roisin Roisin Sullivan O'Callaghan, Benjamin Benjamin O'Callaghan Smith, Calvin Calvin Smith Surana, Sunaina Sunaina Surana Beato, Marco Marco Beato Brownstone, Rob Rob Brownstone <![CDATA[Validation of mouse model: qPCR and Western blots probing for Tor1a-torsinA]]> Neurosciences not elsewhere classified qPCR Western blot Mouse model Neuroscience movement disorder dystonia DYT1 spinal cord 2023-03-27 2023-03-27 Dataset 2023 University College London Model validation dataset associated with Pocratsky et al 2023, Science Translational Medicine


Data generated from postnatal day 18 littermate control (Cdx2::wt;Tor1a-wt/frt) and spinal Tor1a d-cko mice (Cdx2::FlpO;Tor1a-frt/frt) that develop early onset generalised torsional dystonia. Whole tissues were snap frozen and processed for expression of Tor1a and torsinA protein. Raw images of Western blots provided


Validation_qPCR.xlsx - dataset: Tor1a expression in brains and lumbar spinal cords


Validation_Westerns.xlsx - dataset: torsinA expression in brains and lumbar spinal cords


Western_metadata: details for attached images of blots


Full methodological detail provided in manuscript

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