2026-05-17T12:18:36Z https://api.figshare.com/v2/oai
oai:figshare.com:article/13402301 2022-01-04T11:08:10Z category_24700 category_27241 portal_549 item_type_3 month_year_01_2022
10.5522/04/13402301.v1 https://figshare.com/articles/dataset/High_Magnification_Z-Stacks_from_Blood_Films/13402301 https://ndownloader.figshare.com/files/25809704 https://ndownloader.figshare.com/files/25809977 https://ndownloader.figshare.com/files/25810187 https://doi.org/10.1002/jemt.20092 Manescu, Petru Petru Manescu Elmi, Muna Muna Elmi Bendkowski, Christopher Christopher Bendkowski Zajiczek, Lydia Lydia Zajiczek Shaw, Mike Mike Shaw Claveau, Remy Remy Claveau Brown, Biobele J Biobele J Brown 0000-0001-6981-9872 Fernandez-Reyes, Delmiro Delmiro Fernandez-Reyes 0000-0001-5070-9198 <![CDATA[High Magnification Z-Stacks from Blood Films]]> Medical parasitology Other health sciences not elsewhere classified blood film microscopy image fusion extended depth of field z-stack high magnification Medical Parasitology Medical and Health Sciences not elsewhere classified 2022-01-04 2023-05-30 Dataset 2022 University College London All biological samples were collected from participants recruited under the auspices of the CMRG at the 800-bed tertiary hospital, UCH in the city of Ibadan, Nigeria, after after de-identification of the patient information. We trained and tested the CAMI-Fusion network on three different types of samples: (a) Giemsa-stained thick blood films (TBF) and (b) Giemsa-stained peripheral blood smears (PBS) for malaria parasite detection as well as (c) Wright-stained Bone Marrow Aspirates (BMA) for diagnosing blood cancers.


Image acquisition and pre-processing:

We used an upright brightfield microscope (BX63, Olympus, 100W halogen bulb light source) with a 100X/1.4NA oil immersion objective (MPlanApoN, Olympus) and a color digital camera (Edge 5.5C, PCO) to acquire multiple high-resolution z-stacks with an axial step of a 0.5 µm. White balancing was applied to each focal plane image. For each sample acquisition, an empty field of view (empty region of the slide not containing any blood cells) was acquired and considered as a white object reference.

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